Enhanced biocatalysis of phenanthrene in aqueous phase by novel CA-Ca-SBE-laccase biocatalyst: Performance and mechanism

Enzymes-mediated biocatalysis is believed to be a promising strategy for the degradation of polycyclic aromatic hydrocarbon (e.g., phenanthrene) in aqueous phase, due its high efficiency and environmental harmfulness. However, reusability enzymes-mediated requirement redox mediators limited application. In this paper, enhanced phenanthrene removal was achieved by using novel biocatalyst which coencapsulating laccase natural mediator soybean meal extract on calcium modified chitosan-alginate (CA-Ca-SBE-laccase) beads with network pore structure. High 94.4 % after treating solutions 0.5 ppm 20 min CA-Ca-SBE-laccase beads, 20–30 higher than that free ordinary immobilized beads. Both radical pathway oxygen-containing stable radicals nonradical laccase-SBE combined substrates potential generated contributed phenanthrene, maximum double synergy laccase. Benefiting from redox-mediating encapsulated SBE Ca modification chitosan, maintained fairly catalytic activities over wide range pH values, considerable repeatability storage stability were obtained. This study put forward an efficient laccase, support cofunctionalization technology, would provide new perspective increasing application scope biocatalysis.